mouse monoclonal type i il 1 receptor il 1ri  (Santa Cruz Biotechnology)

Journal: The American Journal of Pathology

Article Title: IL-1β Stimulates Brain-Derived Neurotrophic Factor Production in Eutopic Endometriosis Stromal Cell Cultures

doi: 10.1016/j.ajpath.2018.06.011

Figure Lengend Snippet: IL-1β–induced changes in brain-derived neurotrophic factor (BDNF) were mediated through the IL-1 receptor and occurred, in part, at the level of mRNA expression (real-time quantitative PCR). Eutopic endometriosis stromal cells (EESCs) were preincubated with 1 μg/mL IL-1ra for 1 hour before stimulation with 10 ng/mL IL-1β. A: IL-1ra neutralized the stimulation of BDNF by IL-1β. When the gels were over-run, resolution of the 32- and 14-kDa bands into doublets was noted. All three major isoforms were affected. β-Actin levels were not altered. Similar findings were noted in independent EESC preparations. B: Triplicate EESC cultures were incubated for 24 hours in the presence of 0 to 160 ng/mL recombinant IL-1β. Total RNA extracted from the cultures was reverse transcribed and subjected to quantitative PCR amplification. The BDNF data were normalized to glyceraldehyde-3-phosphate dehydrogenase mRNA levels, using the formula 2ΔΔct. Similar findings were noted in independent EESC preparations. n = 3 independent EESC preparations (A and B). ∗P < 0.05 versus control (analysis of variance with Scheffé’s post hoc test).

Article Snippet: Kinase Inhibitor Experiments IL-1ra, a natural inhibitor of type I and II IL-1 receptors, 25 was purchased from R&D Systems (Minneapolis, MN) and used as described previously.

Techniques: Derivative Assay, Expressing, Real-time Polymerase Chain Reaction, Incubation, Recombinant, Reverse Transcription, Amplification

Journal: The American Journal of Pathology

Article Title: IL-1β Stimulates Brain-Derived Neurotrophic Factor Production in Eutopic Endometriosis Stromal Cell Cultures

doi: 10.1016/j.ajpath.2018.06.011

Figure Lengend Snippet: IL-1β–induced changes in regulated on activation normal T cell expressed and secreted [RANTES; chemokine (C-C motif) ligand 5] secretion. Triplicate eutopic endometriosis stromal cell (EESC) cultures were incubated for 24 hours without (control) or with IL-1β. A: Preincubation with a 100-fold molar excess of IL-1ra completely prevented IL-1β–induced RANTES secretion. B: Preincubation with the NF-κB inhibitor SN50 afforded a 34% suppression of IL-1β–induced RANTES, but there was no inhibition (<2%) with the inert peptide SN50M. The c-Jun N-terminal kinase (JNK) blocker, SP600125, inhibited IL-1β–induced effects on RANTES by 79% [P < 0.05, analysis of variance with Scheffé’s post hoc test (all conditions were different from IL-1β except SN50M + IL-1β)] (C). Similar findings were noted in independent EESC preparations. n = 3 independent EESC preparations (C).

Article Snippet: Kinase Inhibitor Experiments IL-1ra, a natural inhibitor of type I and II IL-1 receptors, 25 was purchased from R&D Systems (Minneapolis, MN) and used as described previously.

Techniques: Activation Assay, Incubation, Inhibition